Welcome to your metabolic measurement and metabolic phenotyping resource!

COURSE ANNOUNCEMENT: The next course in metabolic measurement will be held in lovely Las Vegas in April 2017. It will teach participants how to use indirect calorimetry in metabolic screening. We will apply techniques for measuring real-time O2 consumption, CO2 production and water vapor loss for laboratory rodents. Measurement systems also include food & water intake, body mass monitoring, wheel running and voluntary activity. Data analysis - such as extraction of REE and AEE, analysis of circadian changes in EE, uptake and behavior, correlation between activity and EE, and much, much more - is a significant focus of the these courses. Click here for more!

Update! As part of the metabolic phenotyping course held at Sable Systems International three years ago, I introduced a startling new development - a multiplexed Promethion metabolic phenotyping system capable of obtaining excellent VO2 and VCO2 readings from successive cages in under fifteen seconds apiece. Thus, unlike Promethion's competitors in the field of metabolic phenotyping, you won't have to wait about 45 minutes for successive readings from a given rat or mouse in a 16-cage system. Instead, you'll get a new reading, a.k.a. cycle time, up to every two minutes - at least a twenty-fold improvement in throughput speed. This is achieved by the wickedly fast dwell time of under 15 seconds, by a number of trade secrets, and by splitting the analytical workload into 8-cage segments. Thus, you get the same record-breaking 2-minute cycle time whether you have 8, 16, 24, 32, or even 48 or 64 cages. This is in addition to second by second acquisition of sensor data from each cage in the system.

Of course, if you have a real need for speed, a two minute cycle time is intolerable. In that case, Promethion also offers real, field-proven, up-and-running, non-vaporware (get the picture?) systems that update VO2 and VCO2 for every cage every second. These feats are only possible because Sable Systems designs and manufactures all of the equipment used in Promethion systems - the gas analyzers, optical benches, mass flow controllers, data acquisition systems, the works. Everything is optimized for reliability, accuracy, and the exacting resolution required for metabolic measurement. By Scientists, For Scientists, not by consulting engineers for non-scientists. Seriously, nothing in the field of metabolic phenotyping compares to Promethion in terms of resolution, advanced features and the revolutionary "data field system" concept. 

This is the companion web site to the book "Measuring metabolic rates: A manual for scientists", by John Lighton. The publisher is Oxford University Press. Of course, nothing is ever perfect, and this book is no exception. Click here for errata - and if you find any errors, please contact John

This web site is more than a book companion. It features detailed information on several areas of respirometry that John had no room to cover in his book. Among these are metabolic rate and other calculators, plus a series of articles in the blog on some of the latest and most controversial breakthroughs in metabolic measurement. Such as, a massive paradigm shift in metabolic screening systems made possible by "massively parallel" metabolic measurement engines and, if you can't afford those, by wickedly fast multiplexed systems. Many of the blog posts are influenced by the R&D component of John's day job as president of Sable Systems International. This is for your benefit. John also tweets discontinuously and can be followed on ResearchGate should you so desire. You have been warned.

Join in the conversation! If you are a scientist and become a user of this site (see to the left), you can comment on blog postings and interact with John and other users. It's fast, simple, and we don't spam, ever.

If you don't own the book yet, or would like to replace a copy your colleague refuses to return or pretends they've lost, you can purchase one directly from Amazon.com. If you prefer, you can buy an inscribed, signed copy of the book for $59.95 (postage free in the USA, at cost for the rest of the world) complete with errata list from John directly. Don't include credit card numbers - you will be contacted and a secure transfer will be organized. 

Any questions or comments? Please direct them to John via john@johnlighton.org.

Come back soon!

Other news: Do catch up with the site's blog. Friends love it. Enemies hate it. I must be doing something right.



This book is vital reading

John Lighton has probably done more to modernize and consolidate the field of whole-animal respirometry than any single person. And now he has written a book that explains all. I only wish that I had such a reference when I was in graduate school. This book is vital reading for anyone who does or aspires to do respirometry, but it is imperative for anyone who would trust a turn-key system for their data collection. It can save you from the perils of practicing respirometry without a license.

Theodore Garland, Jr
UC Riverside

Your Stories

Why not share some of your respirometric triumphs and disasters? We promise to treat the two imposters just the same - there's something to be learned from failure and frustration, often more than is offered by easy success. Start thinking about ways you can communicate your experiences, good and bad, with metabolic rate measurement. Before long you'll have a chance to share those experiences on this site.